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Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid.

Potapenko DI, Bagryanskaya EG, Grigoriev IA, Maksimov AM, Reznikov VA, Platonov VE, Clanton TL, Khramtsov VV

International Tomography Center, Novosibirsk 630090, Russia.

A new method of measurement of thiol concentration by 19F NMR spectroscopy is developed. The method is based on the detection of products of the exchange reaction of thiols with a newly synthesized fluorinated disulfide, 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid (BSSB). A significant broadening of the 19F NMR signal of BSSB in the presence of thiols was observed and attributed to the exchange reaction between the parent disulfide and 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid. The rate constant for this reaction was found to be equal to (63 +/- 11) x 10(3) M(-1) s(-1) at pH 7.0. The method was applied for the measurement of concentration of glutathione and albumin in rat blood.

Published 3 October 2005 in Magn Reson Chem, 43(11): 902-9.
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