NMR Research Today is a free monthly online journal that collates and summarizes the latest research about NMR, including details on nuclear magnetic resonance, structural determination, techniques.

SAR by ILOEs: an NMR-based approach to reverse chemical genetics.

Becattini B, Pellecchia M

The Burnham Institute, 10901 North Torrey Pines Rd. La Jolla, CA 92037, USA.

Reverse chemical genetics is an emerging technique that makes use of small molecule inhibitors to characterize how a protein functions. In this regard, we have developed an NMR-based approach (SAR by ILOEs) that enables the identification of high affinity ligands for a given protein target without the need of a specific assay. Our approach is of general applicability and could result very powerful in reverse chemical-genetics studies, target validation, and lead discovery. We report a recent application on the design and synthesis of compounds that inhibit protein-membrane interactions.

Published 15 March 2006 in Chemistry, 12(10): 2658-62.
Full-text of this article is available online (may require subscription).

© 2005-2008 NMR Research Today. All Rights Reserved.